How HPLC Columns Work
This Phenomenex animation provides a simple, easy-to-understand overview of how HPLC columns work in chromatography and why they remain essential tools in analytical science.
What is High Performance Liquid Chromatography (HPLC)?
HPLC is a form of column chromatography where a sample mixture (analyte) is pumped through a column packed with stationary phase using a solvent system known as the mobile phase. Separation occurs based on how each analyte interacts with the mobile and stationary phases, producing reliable, high-quality chromatographic results.
What is Ultra-High Performance Liquid Chromatography (UHPLC)?
UHPLC technology uses smaller particle sizes under 2 µm to achieve higher efficiency, faster separations, improved resolution, and greater sensitivity. These smaller particles require instruments capable of handling extremely high backpressure and optimized system volumes to maintain performance. As a result, UHPLC instruments were specifically engineered for these demanding conditions.
UHPLC Performance on an HPLC System
Because UHPLC instruments are costly, many scientists want UHPLC-level performance on their existing HPLC systems. Phenomenex addressed this need by introducing 2.6 µm Kinetex Core-Shell Technology columns in the late 2000s, delivering UHPLC-like efficiency, speed, and resolution on traditional HPLC platforms.
Want to learn more about HPLC, columns, stationary phase, or mobile phase?
Explore these resources:
📄Blog: phenomenex.blog/2017/06/13/what-is-hplc-and-how-does-it-work/?utm_campaign=youtubetracker&utm_source=youtube&utm_medium=social
🌐HPLC Solutions: https://www.phenomenex.com/techniques...
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