Transcriptome Analysis of S. meliloti PHB Mutants - mSystems®
The ability of bacteria to store carbon and energy as intracellular polymers uncouples cell growth and replication from nutrient uptake and provides flexibility in the use of resources as they are available to the cell. The impact of carbon storage on cellular metabolism would be reflected in global transcription patterns. By investigating the transcriptomic effects of genetically disrupting genes involved in the PHB carbon storage cycle, we revealed a relationship between intracellular carbon storage and nitrogen metabolism. This work demonstrates the utility of combining transcriptome sequencing with metabolic pathway mutations for identifying underlying gene regulatory mechanisms.
Doi: https://doi.org/10.1128/mSystems.0003...
Title: Transcriptome Analysis of Polyhydroxybutyrate Cycle Mutants Reveals Discrete Loci Connecting Nitrogen Utilization and Carbon Storage in Sinorhizobium meliloti
Authors: Maya D’Alessio (http://orcid.org/0000-0003-2418-9007)
Ricardo Nordeste
Andrew C. Doxey (http://orcid.org/0000-0003-2015-099X)
Trevor C. Charles (http://orcid.org/0000-0002-0344-5932)
Editor: Jeff Tabor, Rice University
Published in mSystems® on 12 September 2017
mSystems® publishes preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems® encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems® welcomes submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems® provides streamlined decisions, while carrying on ASM's tradition of rigorous peer review.
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