3-D IHC: Mouse spleen - CD3, CD169 (Siglec-1)
Formalin-fixed, 300 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with the following:
Red: Alexa Fluor® 594 anti-mouse CD3 (clone 17A2) - https://www.biolegend.com/en-us/produ...
Green: Alexa Fluor® 647 anti-mouse CD169 (Siglec-1) (clone 3D6.112) - https://www.biolegend.com/en-us/produ...
All antibodies were used at 5 µg/mL. Tissues were optically cleared, then analyzed at 225 µm imaging depth on a confocal microscope.