PCR primer design using NCBI Primer-BLAST
00:00 Introduction
02:06 Practical
To achieve a good primer design process. I recommend NCBI Primer-BLAST tool for this process. It achieves a great result with different levels of conformation.
Primer-BLAST: https://www.ncbi.nlm.nih.gov/tools/pr...
Use Primer-BLAST to check primer specificity: • Primer BLAST: Check your primers specifici...