The Spatiotemporal Organization, Dynamics, and Function of Virus Replication Centers during Murine
Presented By:
Douglas Peters, PhD and Alan Mullan, PhD
Speaker Biography:
Douglas Peters has a BA in Neuroscience and Biology from Augustana College in Rock Island, Illinois, and a PhD in MCDB from the University of Colorado Boulder.
Alan Mullan: My background in microscopy is built from my work and studies while at Queen's University Belfast. I always had a keen interest in bacteria and viruses as pathogens, as well as how we may exploit them for our benefit, so naturally specialised in microbiology.
Webinar:
The Spatiotemporal Organization, Dynamics, and Function of Virus Replication Centers during Murine Polyomavirus Infection
Webinar Abstract:
Small DNA viruses require both host DNA replication and repair factors for their replication. The host cell replication factors are often recruited by the virus to subnuclear domains referred to as viral replication centers (VRCs). Several factors have been immunolocalized to VRCs using fluorescence microscopy, but little else is known about VRC spatiotemporal organization or dynamics. We investigated the organization and function of VRCs during murine polyomavirus (MuPyV) infection using 3D structured illumination microscopy (3D-SIM). We immunolocalized viral and cellular VRC components to spatially distinct VRC subdomains through which viral DNA (vDNA) trafficked sequentially post-synthesis, suggesting these VRC subdomains have distinct functional roles in vDNA processing. We also observed the disruption of VRC organization and function during mutant MuPyV infections or inhibition of DNA synthesis. These results reveal a dynamic organization of VRC components that coordinates MuPyV replication during infection.
Earn PACE Credits:
1. Make sure you’re a registered member of LabRoots (https://www.labroots.com/ms/webinar/s...)
2. Watch the webinar on YouTube or on the LabRoots Website (https://www.labroots.com/ms/webinar/s...)
3. Click Here to get your PACE credits (Expiration date: October 08, 2022): (https://www.labroots.com/credit/pace-...)