Continuous Protein Monitoring using Ocean HDX
Learn more about continuous, online monitoring of proteins using UV absorbance. Ocean Optics offers robust, scalable sensing tools, and application knowledge, design and engineering expertise, to help manufacturers navigate process challenges. See how we use an Ocean HDX high-sensitivity spectrometer to capture real-time protein concentration values, and then convert the data into answers that are meaningful to the user.
Continuous protein monitoring and benefits
One of the most powerful uses of spectroscopy is its ability to perform continuous monitoring of some process — at the lab scale or the industrial scale. Ocean Optics provides a reliable, quantitative measurement system that never goes home. You can think of it like having someone on your QC line that's always looking perfectly at your product, and who is available 24/7 to give you reliable answers.
It's essential to set up a spectral system in a way that can continuously monitor your sample rather than just take point measurements. This is what we're looking at in the protein monitoring demo — specifically, a microfluidic setup using one of our FIA Flow Cells. The concepts illuminated with microfluidics hold true at the industrial level as well.
Overview of the demo results and our findings
The sample we examine is BSA, or bovine serum albumin, a common protein that can be used as a substitute for other, more serious compounds. By building a Schematic in OceanView to dynamically output grams per liter of BSA without doing on-the-fly calculations by hand, we demonstrate how to quickly convert an absorbance into a concentration value automatically using our software.
Lineworkers and technicians no longer have to look at wavelengths, counts, absorbance values and intensities to view pertinent information. In sum, we examine the power of setting up these dynamic flowing systems and building a schematic in such a way that it boils the spectroscopy into a meaningful output.
Timestamps:
[0:17] Using spectroscopy for continuous monitoring
[0:54] Protein monitoring demo looking at a microfluidic setup using one of our FIA Flow Cells
[2:06] Dynamic flowing system featuring bovine serum albumin (BSA) in aqueous solution and building an OceanView Schematic to turn that absorbance value into concentration quickly
[2:26] Equipment used includes a light source (280 nm UV LED) LSM-280A and a light source controller LDC-1, a PEEK Flow Cell, microfluidics and a FIA-PUMP-C Fluidics Pump, and the Ocean HDX spectrometer
[4:29] Spectroscopy techniques and how this equipment works in the software
[9:12] What happens when we change the tubing to our first concentration of approximately 2 grams per liter of BSA
[11:08] Turning this sample into a concentration value with OceanView Schematic
[16:32] A more dilute sample to see the absorbance and BSA concentration drop
[18:05] Build a concentration output using the OceanView Schematic, as well as track a dynamic flowing system using Applied Spectral Knowledge
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