Hear Tamina Lebek, a PhD student at the University of Edinburgh, talk about the PUFFIN system (Positive Ultra-Bright Fluorescent Fusion for Identifying Neighbours).
This cutting-edge method expands the tool kit for cell labelling, making it easier to study how cells communicate during development.
The PUFFIN system uses a highly charged fluorescent protein to label neighbouring cells, allowing researchers to study cell communication, differentiation, and interaction in a variety of organisms. Tamina explains how the PUFFIN system can be applied to different cell types and organisms, including chicken, mouse, zebrafish, and flies.
It offers a versatile approach to identifying secretor cells and their neighbours using microscopy and flow cytometry, using only a single plasmid.
Learn how this system can unlock new insights into cellular behaviour during development.
Read her research paper here: https://www.embopress.org/doi/full/10...