Trimmomatic tutorial 🛠️Install 💻 Run 🎓Single End 🎓 Paired end
Trimmomatic tool link
http://www.usadellab.org/cms/?page=tr...
System configuration
Processor- Intel i5 4th gen, 1.7 GHz, 4 CPU(or thread)
RAM-8GB
Boot and working drive- 256GB SSD
OS- Linux Mint 21.1 Cinnamon
------------------------------- Follow Transcriptome analysis pipeline ------------------------
------ Tutorial made the year 2024-2025
------ Learn Download fastq(raw file-SRA) file from NCBI
• SRA tool kit || installation to SRA file d...
• SRA to fastq file conversion || SRA toolki...
------ Learn Fastq file quality check
• Fastp | Install | Run | Paired end #bioinf...
------ Learn fastq to Bam file generation
• Bowtie2 | Install | Build Index | Alignmen...
------ Learn the SAM to BAM file sort by Samtool
• Samtools | Install | SAM to BAM conversion...
------ Learn BAM file to Count matrix generation
• Featurecount | Install | BAM to Count matr...
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