Ask a Scientist: What is Fold-80 Penalty?
What is the Fold-80 base penalty score in target-enriched next-generation sequencing (NGS)? This video describes the relationship between the Fold-80 base penalty score and coverage uniformity, and how understanding this metric can help conserve sequencing resources. It also explains important differences between specificity and uniformity (as they relate to NGS target coverage), and provides tips on how to improve the Fold-80 score in hybridization-based target enrichment workflows.
Presented by Teri Mueller of Roche Sequencing and Life Science.
MC-US-08233