Ask a Scientist: What is On-Target Rate in NGS?
What is on-target rate in next-generation sequencing (NGS), and how does it relate to the efficiency of target enrichment? This video explains the differences between % reads on-target and % bases on-target, as well as how the inclusion of padding prior to data analysis can impact these sequencing metrics. It also offers tips that can be implemented at several steps of the hybridization-based target enrichment workflow—from probe selection to data analysis—to improve on-target rates.
Presented by Dustin Masser, Roche Sequencing and Life Science
MC-US-08235