Ask a Scientist: How does the 20% Rule Help Manage Expectations in Targeted NGS?
What is the 20% rule in NGS Target Enrichment? This video explains why sequencing to a coverage depth greater than 20% of the theoretical maximum coverage for a sample may lead to an increased rate of PCR duplicates. It also describes how each aspect of the library prep workflow—including shearing method, kit choice, size selection, and multiplexing scheme—can influence the accuracy of final coverage estimates.
Presented by Teri Rambo Mueller, Field Applications Consultant at Roche Sequencing and Life Science.
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