Calcium-activated chloride channel TMEM16A
Supplementary video from the paper "Activation mechanism of the calcium-activated chloride channel TMEM16A revealed by cryo-EM," authored by Cristina Paulino, Valeria Kalienkova, Andy K. M. Lam, Yvonne Neldner & Raimund Dutzler, published in Nature 552, 421–425 (21 December 2017)
http://dx.doi.org/10.1038/nature24652
Video 1. Ligand-bound mTMEM16A structure. cryo-EM density map of the mTMEM16A ion channel obtained in presence of calcium ions with the modelled structure superimposed. Only one subunit is shown and the two bound Ca2+ are coloured in blue.
Video 2. Ligand-free mTMEM16A structure. cryo-EM density map of the mTMEM16A ion channel obtained in absence of calcium ions with the modelled structure superimposed. Only one subunit is shown.
Video 3. Comparison of the Ca2+-bound and Ca2+-free state.
Superimposition of the mTMEM16A ion channel structure in the Ca2+-bound (green) and the Ca2+-free (violet) state.
Video 4. Ca2+-induced conformational changes. Morph between the Ca2+-bound and the Ca2+-free state (violet) superimposed on the structure in presence of calcium (green, Ca2+ shown as blue spheres).