The principle and how to work thin layer chromatography (TLC) animation
The principle and how to work thin-layer chromatography?
Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase), Solids most commonly used in chromatography are silica gel (SiO2 x H2O) and alumina (Al2O3 x H2O).
Simple processing Plate TLC inside chamber TLC and will solvent rises plate TLC. Spots begin views to form following the solvent.
To visualize developed TLC plates, generally placing under ultraviolet or UV light. Silica plate will appear fluorescence under UV light, compound samples and standards will be seen as dark spots.
The identified sample has calculated the value of rF value
rF value is the distance moved by spot divided distance moved by solvent
Compare the value of rf sample with value rF standard
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