Botrytis bunch rot (Botrytis cinerea), powdery mildew (Erysiphe necator) and downy mildew (Plasmopara viticola) cause significant economic impact in vineyards and management of these diseases can be compromised by resistance to fungicides. Laboratory and field resistance to key fungicides has been detected in all three pathogens in many Australian viticultural regions. Early detection of resistance is critical for effective management of this problem. Laboratory based phenotype and genotype diagnostic techniques have been developed to determine resistance. The results are being used to progress to real-time, in-field detection as well as high-throughput laboratory testing of resistance for several fungicide groups with known resistance mechanisms. This webinar will provide an update on 1. resistance detection and monitoring research, 2. sensitivity of existing and new fungicides across viticultural regions, and 3. the optimisation of in-field sampling methods.