Usually problems with Alpkem/OI Analytical Autoanalyzers, whether Segmented Flow (SFA) or Flow Injection Analysis (FIA), are related to that which falls under the direct control of the operator such as water, reagents, standards, or basic instrument configurations such as pump tubes or luer connections.
When troubleshooting we should always go from the most likely and simple aspects of the system to the more complex hardwired components. In my experience once an Autoanalyzer instrument is working it tends to work until something is done to make it not work; an ongoing preventative maintenance program especially helps to make this true.
In this video we will address the importance of establishing a quiet and flat baseline on DI water before starting your analysis.