Contractility Algorithms for Primary and Cultured Myocytes
Contractile performance in cardiomyocytes has long served as a key measure of cardiac response to compound treatment as well as genetic modification and pathophysiological conditions. IonOptix offers several contractility algorithms suited to different cardiomyocyte preparations. In this comparison video, we identify the appropriate algorithm to use for primary or cultured cells, as well as specific use cases for each. We also illustrate usage and best practices while describing some of each algorithm's underlying technology. If you're interested in more information, please contact us at [email protected], or click the following links for our contractility systems...
MultiCell High Throughput System
https://www.ionoptix.com/products/sys...
Calcium and Contractility System
https://www.ionoptix.com/products/sys...
CytoMotion Lite System
https://www.ionoptix.com/products/sys...
For the specific sarcomere length algorithm application notes referenced in the video, click either of the following links...
https://www.ionoptix.com/resource/sar...
https://www.ionoptix.com/resource/sar...
0:00 Introduction
1:55 Sarcomere Length
10:00 SoftEdge (Cell Length) overview
10:28 SoftEdge outside-in edge detection
20:12 SoftEdge box method edge detection
25:22 CytoMotion
32:15 Sarcomere vs Cell Length comparison in primary adult cardiomyocytes
34:47 CytoMotion vs Cell Length comparison in iPSC-CMs
37:12 Summary