Three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet (Part 2)
Example 2 of three-dimensionally reconstructed images of immunostaining of the mouse ear epidermal sheet prepared via the Basic Protocol 1. Epidermal tight junction (ZO-1 staining in green), Langerhans cells (MHC II staining in red), and dendrites of Langerhans cells and tight junction (claudin-1 staining in blue) visualized by confocal microscopic observation. MHC II of Langerhans cells localizes at cell surface when Langerhans cells are activated. The dendrites of Langerhans cells elongate upward to dock with tight junctions. ZO-1 and claudin-1 accumulate at the docking point.
For more information, please see the associated protocol: Whole-Mount Preparation and Microscopic Analysis of Epidermis
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