Live imaging showing non-enzymatic generation of a monolayer cell culture from MGOs by direct culture of the organoids in Cellstart-CTS coated plates. Histone 3.3G34R MGOs attach to the plate and tumor cells migrate from the parental MGOs through the plate surface generating a 2D culture. Tumor cells express EGFP and tumor microenvironment cells express tdTomato.
For more information, please see the associated protocol: Murine-Derived Glioma Organoids and Cell Line Culture Systems
https://currentprotocols.onlinelibrar...