Conditional colocalization analysis of three-color microscopy images
Khuloud Jaqaman, UT Southwestern Medical Center
I2K 2022 | Talk Session #9 | May 10th
Colocalization analysis is a cornerstone approach in cell biology. However, it is mostly limited to two-color images. Here we developed a novel colocalization analysis approach for three-color microscopy images. Going beyond the question of whether colocalization is present or not, it addresses the question of whether the colocalization between two entities is influenced, positively or negatively, by their colocalization with a third entity.
Authors: Jesus Vega-Lugo, UT Southwestern Medical Center; Bruno da Rocha-Azevedo, UT Southwestern Medical Center; Aparajita Dasgupta, UT Southwestern Medical Center; Khuloud Jaqaman, UT Southwestern Medical Center
Keywords: colocalization, interactions, point-pattern analysis, light microscopy, super-resolution