Keratinocyte cell movements
Keratinocytes were cultured in a 35mm dish and imaged using a DeltaVision CORE microscope, equipped with an incubator and carbon dioxide mixer. The imaging technique used was Differential Interference Contrast (DIC) microscopy. Time lapse images were acquired every 1 second over a period of 10 minutes.
The cells are adhered to the bottom of the culture dish and dynamic movements can be seen within the cells and at the periphery as the cells migrate. Lamellipodia and filopodia can easily be observed.