Keratinocyte cell division
Keratinocytes were cultured in a 35mm dish and imaged using a DeltaVision CORE microscope, equipped with an incubator and carbon dioxide mixer. The imaging technique used was Differential Interference Contrast (DIC) microscopy. Time lapse images were acquired every 1 second over a period of 10 minutes.
The cells have already rounded up as the movie begins and cell division proceeds during the movie. The new daughter cells then settle down to the bottom of the dish (out of focus) and start to migrate.