Incucyte® Tutorial Series: Learn How to Remove Background Fluorescence When Image Processing
Incucyte® Live Cell Analysis Tips and Tricks:
Background florescence can occur due to the cell culture media components or other artifacts in media. Incucyte® software offers integrated image processing techniques—such as Top-Hat and Surface Fit Background Subtraction—to correct for background fluorescence.
This video explores:
Top-Hat Background Subtraction
Surface Fit Background Subtraction
How to Use Autoscale
Key Moments:
0:38 Top Hat Background Subtraction: Top Hat Background Subtraction can be explained by the moving disk concept to determine genuine signal the user defines a disk based on the radius of the largest fluorescent object within an image.
1:43 Surface Fit Background Subtraction: The option to choose the surface fit background subtraction and segmentation algorithm. The user will designate a threshold intensity value without defining object size.
2:15 Auto scale: Autoscale is a fluorescence visualisation tool that chooses the minimum and maximum intensity display settings based on the range of pixel intensities in the images. Vessel Autoscale applies a single scale range to each image based on the minimum and maximum brightness across the entire vessel.
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