Real Time EV Uptake Characterization Using an Incucyte®️ Live Cell Imaging System
Laura Skerlos, a senior scientist at Sartorius, presented research at the International Society for Cell and Gene Therapy conference in Vancouver, Canada. The research team introduced an innovative labeling approach for continuous monitoring and analysis of extracellular vesicle (EV) interactions with cells. They described the use of a covalently bound dye optimized to label EVs for real-time analysis and the Incucyte®️ live cell imaging system.
The validation of the EV labeling kit was performed using Hansa BioMed exosomes and PC3 cells, demonstrating its compatibility and effectiveness. The study also explored the concentration dependence of EV uptake, highlighting the importance of optimizing EV concentrations and experimental setups. The Incucyte Exofluor Green EV labeling kit enables real-time visualization and quantification of EV uptake in live cells with a stable covalently bound dye.
Learn more: https://www.sartorius.com/en/applicat...
Transcript:
00:51 Real Time EV Uptake Characterization Using an Incucyte®️ Live Cell Imaging System
02:26 Exofluor Green Labeling Method
03:33 MSC-Derived EV Purification Process
04:53 Validation of EV Labeling Kit
09:27 Scratch Wound Migration
#Incucyte #ScratchWound #ExtracellularVesicles