Genomic library | DNA library | class 12
Aslam o alikum
I am Hassam ur Rahman and I am teaching Fsc biology since 2014 .I am always trying to improve myself and provide best lecture to students.i am taking content for the lecture from authentic and relevant sources but human errors are possible . you are requested to please highlight the mistakes.My lectures are equally reliable for Fsc and mdcat students
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This lecture is about
Genoiftic Library
A genome is a full set of genes of an individual. A genomic library is a collection of
bacterial or bacteriophage clones, each clone containing a particular segment of
DNA from the source cell. For making a genomic library, an organism’s DNA is simply
sliced up into pieces, and pieces are put into vectors (i.e. plasmids or viruses) that are
taken up by host bacteria as shown in Fig. 23.3. The entire collection of bacterial or
bacteriophage clones that result contains all the genes of that organism.
A particular probe can be used to search a genetic library for a certain gene. A probe
is a single stranded nucleotide sequence that will hybridize (pair) with a certain piece
of DNA. Location of the probe is possible because the probe is either radioactive or
fluorescent. Bacterial cells, each carrying a particular DNA fragment, can be plated onto
agar in a petri dish. After the probe hybridizes into the gene of interest, the genes can
be isolated from the fragment (Fig 23.4). Now this particular fragment can be cloned
further or even analyzed for its particular DNA sequence.