Practical exploration of morphological profiling data (Morphological profiling, part 2)
A practical discussion of how profiling experiments are analyzed by bioimage analysts using Morpheus. If you want to better understand the theory and the data treatment that takes place prior to this video, please watch part 1 at • The beginner's guide to morphological prof...
Helpful links (for both videos):
The written tutorial, including all the data shown in parts 1 and 2 - https://github.com/broadinstitute/BBB...
How to determine if your segmentation is "good enough" - https://carpenter-singh-lab.broadinst...
A blog post on considerations of normalization - https://carpenter-singh-lab.broadinst...
The CellPainting protocol - https://www.biorxiv.org/content/10.11...
A multi-lab paper describing morphological profiling methods - https://www.nature.com/articles/nmeth...
The "Dinosaurus Dozen", aka why you should always directly examine your data in addition to doing high-level exploration in tools like this - https://www.autodesk.com/research/pub...
An introduction to t-SNE clustering (video 2) https://en.wikipedia.org/wiki/T-distr...
The original paper describing the data set used in both videos - https://slas-discovery.org/article/S2...
The paper describing Cell Painting discovering novel pathway biology (video 1) - https://elifesciences.org/articles/24060
A recent review on what Cell Painting and other phenotypic drug discovery methods can do - https://www.nature.com/articles/s4157...