BIT:5 why endonucleases do not cut their own DNA| why sticky ends are preferred
Aoa! MY name Is Ehsan and welcome to Biology with Ehsan
In this lecture we discussed important questions about restriction endonucleases enzymes during recombinant DNA technology.
1) why restriction endonucleases do not cut their own bacterial DNA?
2) Why sticky ends/cohesive ends are preferred in genetic engineering?
3) what is the relation of DNA fragments and number of restriction sites?
4) How many DNA fragments of circular DNA will be produced if number of restriction sites is one?
5) How many DNA fragments of linear DNA will be produced if number of restriction sites is one?
6) How many types of restriction endonucleases?
7) How bacteria modify it's DNA to protect itself form restriction endonucleases?
8) What is DNA methylation?
9) what is restriction modification system?
10) How bacteria protect itself from restriction endonucleases enzymes?
11) what is the defense mechanism of bacteria against viral attack?
And many such questions are addressed in the lecture.
Lecture is equally important for all biology students especially for federal board biology second year students.
0:00 Introduction
02:07 Why restriction endonucleases do not cut their own DNA?
13:22 Why sticky ends are preferred in genetic engineering?
21:00 Relation of DNA fragments and number of restriction sites for endonucleases
#biotechnology
#biology
#bioscience
#biologyclass12
#recombinantdnatechnology
#rdnatechnology
About:__________________________
Name: Ehsanullah
University: Graduated from Quaid e azam university Islamabad, Pakistan
Department: Department of Biotechnology, Quaid e azam university Islamabad
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