MULTI-PHOTON - Z-Stack of 3D nano-printed scaffold imitating native lung tissue
This amazing multi-photon z-stack of 3D nano-printed scaffold imitating native lung tissue sourrounded by lung cells, made with our MPX-1040 microscope (20x, 0.75 NA, FOV 600 µm, z-stack: stepsize 3 µm).
Overlay of 2P-460 nm (Hoechst, blue), 2P-542nm (Rose Bengal, green) and 2P-595 nm (Phalloidin-ATTO-490LS, red).
You can see the cell nuclei (blue), scaffold structure (green) and the stained actin filaments of A549 lung cells (red). Cells were kindly provided by Amelie Erben supervised by Dr. Stefanie Sudhop (CANTER - Hochschule München).