This image shows a cross section of a mouse colon imaged from the whole organ with our multi-photon setup: Healthy FA-fixed mouse colon stained with Hoechst (16x, 0.8 NA, FOV 2.7 x 3.8 mm, pixel size: 0.32 µm) - overlay of Hoechst 460 nm (blue), SHG-515 nm (green), autofluorescence 595 nm (red).